Transgressive resistance to <i>Heterodera glycines</i> in chromosome segment substitution lines derived from susceptible soybean parents

Abstract Chromosome segment substitution lines (CSSLs) are valuable genetic resources for quantitative trait loci (QTL) mapping of complex agronomic traits especially suitable minor effect QTL. Here, 162 BC 3 F 7 –BC CSSLs derived from crossing two susceptible parent lines, soybean [ Glycine max (L.) Merr.] ‘Suinong14’ (recurrent parent) × wild ( G. soja Siebold &amp; Zucc.) ZYD00006, were used QTL cyst nematode (SCN, Heterodera glycine Ichinohe) resistance based on female index (FI) and cysts per gram root (CGR) through phenotypic screening whole‐genome resequencing CSSLs. Phenotypic results displayed a wide range distribution transgressive in both HG Type 2.5.7 FI CGR demonstrated higher correlation between weight R 2 = .5424) compared with than .0018). Using the single‐marker analysis nonparametric test, 33 significant detected 18 chromosomes contributing to CGR. Fourteen 5.6–15.5% variance (PVE) revealed 11 chromosomes, 16 accounting 6.1–36.2% PVE 14 strong additive by multiple‐QTL model (MQM) mapping. Twenty‐five 13 out all 38 identified 20 ZYD00006 Suinong14, respectively. The combination positive alleles FI, CGR, exhibited low reproduction. For first time, associated have been detected, should be considered breeding purposes absence genes such as rhg1 Rhg4 .